DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click
HERE to register or log in.
Accession Number:
AD1135306
Title:
Gut and Cartilage Microbiomes as Novel Mediators of Inflammation in and Potential Therapy for Post-Traumatic Osteoarthritis
Descriptive Note:
[Technical Report, Annual Report]
Corporate Author:
OKLAHOMA MEDICAL RESEARCH FOUNDATION OKLAHOMA CITY
Report Date:
2021-02-01
Pagination or Media Count:
15
Abstract:
In this study we plan to evaluate both the pathogenic and potential protective effects of changes in body microbiomes along with their interactions with the immune system in PTOA, using cutting-edge microbiome and immune cell subtyping analyses. In Aim 1 year 1, we will evaluate correlations between gut and cartilage microbiomes in PTOA-susceptible C57BL6J B6 and PTOA-resistant MRLMpJ mice MRL before and after DMM-induced PTOA, and correlate these findings with systemic inflammatory cell responses via peripheral blood cell phenotyping. In Aim 2 year 2, we will establish the direct contributions of the gut microbiome in both establishment of a cartilage microbiome and in PTOA pathogenesis by introducing the gut microbiota of B6 and MRL mice into germ-free GF mice, followed by DMM. In Aim 3 year 2, we will examine the potential therapeutic potential of altering the gut microbiota as a preventative therapeutic for PTOA by conducting gut microbiome transplantation of superhealer MRL into non-healer B6 mice, measuring susceptibility to OA, and quantifying associated systemic inflammatory cell changes.
Distribution Statement:
[A, Approved For Public Release]
