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Novel Artificial Erythrocyte for In-Field Resuscitation of Hemorrhagic Shock

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[Technical Report, Annual Report]

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University of Maryland School of Medicine

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The first ErythroMer prototype EM-V1 was structurally stable, toroidal size 17510nm pdi 0.260.0 by DLS, confirmed by TEM and AFM. CH50 complement activation results were indistinguishable from negative controls, and we observed no impact on plasma viscosity110 and 15 dilution with EM-V1. For the most recent variant of EM-V1 with increased concentration of the allosteric effector, p50 was33.33 Torr control RBC p50 24.76. EM NO sequestration varied with shell crosslinking and was RBCs. Important to the future successful testing of EM in various models of prolonged-field care PFC, we developed and fine-tuned a rabbit hemorrhagic shock model. Initial shock studies in a rodent model 40 blood volume was removed animals were resuscitated with EM-V1 40wtvol , Hb4mM or normal saline N6, each. EM stabilized hemodynamics and following parameters within 1h lactic acidosis 8.22.1 v 3.21.5 mM for EM and NS, respectively, throughout A-VO2 difference 2411 v 6723 and brain pO2 30.51.4 v 17.21.3Torr p0.05, for all. Hemodilution model HIF-1ODDluciferase mice underwent hemodilution 70 vv with pentastarch, bloodautotransfusion, or EM N6, all native Hb target nadir 5 mgdL. HIF-luc radiance was higher with HES than autotransfusion and EM, which did not differ p0.01. We recently transitioned to our second prototype, EM-V2, and have preliminarily structural data size 320nm pdi 0.37 by DLS. We will endeavor to mirror the recent p50 results from our first prototype in Y2 of this grant with EM-V2. Early PK testing with EM-V2 revealed distributiont124.5hrs in rabbits n2. In rabbit oxygenationacute shock studies, sufficient blood volume BV was removed to induce an increase in lactate, decrease in liver pO2 and decrease in mean arterial pressure animals were resuscitated with EM 30 BV, N3 or 5 Albumin or Auto-transfusion N5 of whole blood N6

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  • Medicine and Medical Research

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[A, Approved For Public Release]