Direct Imaging of Modulatory Neurotransmitters Using Synthetic Nanosensors to Understand and Treat Parkinson's Disease
[Technical Report, Annual Report]
The Regents of the University of California
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Nanosensors for the optical detection of dopamine in brain slice has been validated in wildtype C57C6 mice as well as a Parkinsons model mouse. Using nanosensors, endogenous dopamine release in striatal brain slice is triggered by electrical stimulation and quantified using microscopy. We have established the workflow for this procedure and have prepared a population of Parkinsons and wildtype mice to image in the coming quarter. We have confirmed that the method can quantitatively distinguish dopamine release between subregions of the striatum, a critical step in confirming the method for use in Parkinsons studies. COVID-19 shutdowns stalled research starting in Mar. Research facilities are reopening and the project is expected to resume this quarter.
- Medicine and Medical Research