Osteoporosis OP is one of the most common diseases affecting aging individuals and vertebral fractures are the most common type of fracture, complicating osteoporosis. Current techniques for assessing bone strength and osteoporotic fracture risk may be inaccurate and ultimately measure surrogates for biomechanical properties. The proposed research idea is based on the digital volume correlation DVC technique, which allows for measurement of displacements inside a porous object such as a vertebra, in response to an applied load. In using DVC combined with digital tomosynthesis DTS, we can measure the in vivo, patient-specific biomechanical response of vertebrae to load. The overall objective of the proposed research is to clinically validate new DTS-based textural and DVC methods for identification of patients at risk of vertebral fractures in clinically significant cohorts.