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Prospective-Retrospective Analysis of PTEN Immunohistochemistry Assay for Prediction of Outcomes in Recurrent and Metastatic Prostate Cancer

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[Technical Report, Annual Report]

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Johns Hopkins University

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As part of the CDMRP-funded Precision Medicine Biomarker Validating Center, we have developed a robust, highly analytically validated and cost-effective immunohistochemistry IHC-based assay to interrogate PTEN loss in prostate cancer. Our PTEN assay is prognostic in multiple cohorts of surgically-treated prostate cancer patients now, we propose to leverage this body of previous validation studies to test the hypothesis that PTEN loss in primary prostate cancer predicts for a less robust response to hormonal therapies, in the context of two recent, practice-changing Phase III clinical trials for which we have CTEP approval to access specimens. Here, we report on progress to date. We have obtained tissue microarrays containing tissues from 306 patients for RTOG96-01 and immunostained them for PTEN and ERG. In initial analyses, patients with intact PTEN have a 50 percent decrease in cumulative incidence of metastasis with combined radiation therapy and anti-androgen therapy compared to radiation therapy alone, while patients with PTEN loss do not see the benefit of additional anti-androgen therapy.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Biochemistry
  • Medicine and Medical Research

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[A, Approved For Public Release]