Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines
Technical Report,01 Sep 2016,01 May 2020
University of Rhode Island Kingston United States
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The objective of this project was to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive T and B cell responses were measured using molecular and cellular approaches, including RNA sequencing, flow cytometry, and multiplex immunoassays. Overall, this project generated a detailed characterization of immune activation and antigen-specific immune responses in subjects from the clinical trial. Analysis of the data has identified candidate early markers of vaccine immunogenicity which are available to be used in future studies of this vaccine, other dengue vaccines, and vaccines against other viral infections.
- Medicine and Medical Research