Effect of HSV-1 Latent Infection on Laser-Induced Axotomy
[Technical Report, Final Report]
Pagination or Media Count:
The crux of the proposed and now completed work was to test the interaction between HerpesSimplex Virus Type I HSV-1 infection and traumatic brain injury. To do this, our work tested the overall hypothesis that HSV-1 would exacerbate neuronal pathology prompted by a model of moderate brain injury. Our approach combined the use of sonic waves to disrupt neuronal structure, acute HSV-1 infection of primary neurons, and imumunodetection of the phosphorylated form of the cytoskeletal protein taua pathological hallmark of Alzheimers Disease. Our results corroborated evidence that HSV-1 increased levels of phosphorylated tau in Vero cellsa kidney epithelial cell line. Additionally, our work showed, for the first time to our knowledge, that HSV-1 infection has an additive effect with sonic wave-induced damage in primary neurons. Taken together, our work from thisgrant period support the notion that the existing virus in the central nervous system will lead to greater neural damage if the nervous system is disturbed by a sonic wave.
- Medicine and Medical Research