Accession Number:

AD1112107

Title:

Sub-Diffraction Temperature Mapping of Protein Interconversions

Descriptive Note:

[Technical Report, Final Report]

Corporate Author:

University of Michigan

Personal Author(s):

• Lee, Somin

Report Date:

2020-09-15

Pagination or Media Count:

15

Abstract:

The ability to spatially and temporally map nanoscale environments would be highly transformative for revealing dynamic biophysical phenomena. As investigations of time-dependent processes demand broad time observation windows previously unreachable by fluorescence super-resolution, a nonbleaching strategy is needed. We developed precision optics to suppress errors required for subdiffraction imaging. We scaled this method to unlimited numbers of scatterers. With a nonbleaching subdiffraction approach capable of broad timescales, we followed the temporal evolution of actin materials with subdiffraction resolution over broad time periods.

Descriptors:

Subject Categories:

• Biochemistry
• Optics

Distribution Statement:

[A, Approved For Public Release]