Sub-Diffraction Temperature Mapping of Protein Interconversions
[Technical Report, Final Report]
University of Michigan
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The ability to spatially and temporally map nanoscale environments would be highly transformative for revealing dynamic biophysical phenomena. As investigations of time-dependent processes demand broad time observation windows previously unreachable by fluorescence super-resolution, a nonbleaching strategy is needed. We developed precision optics to suppress errors required for subdiffraction imaging. We scaled this method to unlimited numbers of scatterers. With a nonbleaching subdiffraction approach capable of broad timescales, we followed the temporal evolution of actin materials with subdiffraction resolution over broad time periods.