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Apyrase: A Portable Treatment to Prevent Burn Progression and Infection

Descriptive Note:

[Technical Report, Annual Report]

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Definitive treatment of burns often requires surgical excision and grafting. However, the facilities and personnel needed for this may not beacutely available in the combat casualty care arena. This creates the need for interim care strategies that would promote healing and preventinfection until more definitive treatment can be provided. Topical apyrase, an adenosine triphosphate ATP hydrolyzing enzyme, has localanti-inflammatory and anti-microbial characteristics that proved beneficial in our preliminary studies. We hypothesize that topical applicationof apyrase to burn wounds will reduce inflammation, minimize wound progression, and eliminate infection without local toxicity. In the firstaim of the study, we developed a porcine model of partial thickness burn injury to compare the effectiveness of two dosages of apyrase witha standard method of treatment and the in vivo work was finished in this annual reporting period. Serial biopsies, wound measurements andphotographs were taken over time to assess inflammation and healing responses. Final results are pending. Work on Specific Aim IIinvolving infected burn wounds began in this reporting period as well. Optimal bacterial growth conditions were defined and inoculum sizedetermined for the infection experiments. Wounds were infected and treatments applied one day after burn and then daily for 4 days. Mostnotably, blinded, assessments of wound characteristics suggest that infected wounds treated with apyrase more closely resemble uninfectedwounds at Day 3 post burn than do infected burns treated with either saline of sulfamylon. The results of culture and biofilm studies willprovide more quantitative assessments and will be available at the conclusion of the in vivo studies.


Subject Categories:

  • Medicine and Medical Research
  • Pharmacology
  • Microbiology
  • Biochemistry

Distribution Statement:

[A, Approved For Public Release]