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Harnessing Single-Cell Technologies to Understand and Diagnose Rejection in Clinical Face and Upper Extremity Transplantations

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Technical Report,30 Sep 2019,15 Apr 2020

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Brigham and Womens Hospital Boston United States

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Monitoring and immunosuppression of patients following vascular composite allograft VCA transplantation is currently based on protocols used in solid organ transplantation SOT. However, VCAs pose unique immunological challenges because they contain a significant load of passenger donor immune cells. A deeper understanding of mechanisms underlying VCA rejection and identification of biomarkers that can reliably distinguish rejection from other skin inflammation are crucial for the development of more selective immunosuppression targeted specifically for VCA recipients. We will retrospectively study cryopreserved allograft skin biopsies from 7 face and 3 upper extremity transplant patients by single nucleus RNA sequencingsNucSeq, to 1 identify the cell type, frequency and gene expression profiles hence, their functional states and phenotypesof graft-derived and infiltrating cells within VCAs during ACR and non-rejection, and 2 compare single nucleus gene expression profiles of ACR with that of non-transplant related skin inflammation to discover unique molecular changes occur within VCA transplants during ACR, which are distinct from non-rejection related skin inflammation. IRB approval for this project has been obtained and HRPO approval is pending.

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  • Medicine and Medical Research

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