Regulatory Mechanisms of Radioresistance under Differential Levels of Ionizing Irradiation
Technical Report,14 Mar 2016,14 Jul 2019
UNIVERSITY OF TEXAS AT AUSTIN AUSTIN United States
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Unraveling mechanisms of cell survival under oxidative stress is important to protect human health and increase human performance in harsh environments. As an extremophile that can withstand unusually high levels of ionizing radiation, Deinococcus radiodurans hides a wealth of strategies to manage oxidative stresses that are not fully understood. The overarching goal of this project was to characterize the function and biological importance of our newly discovered RNA regulators, in the context of ionizing radiation and chemically induced oxidative stress. We proposed to profile some of the most active candidates to investigate new hypotheses that 1 different mechanisms of oxidative stress management are triggered by different levels of radiation, and 2 that previously uncovered regulatory elements i.e. 5UTR remain largely functional during oxidative stress to control transporters that are critical in initially boosting antioxidants while decreasing cellular toxins. The proposed work builds upon our recent discovery of 54 novel potential 5UTR riboswitches that differentially regulate gene expression under relatively high 15 kGy and low 1 kGy ionizing radiation and are largely found upstream of cellular transport genes.
- Radioactivity, Radioactive Wastes and Fission Products
- Anatomy and Physiology
- Genetic Engineering and Molecular Biology