Preliminary Results for the Incorporation of Peptides into the Traditional Lateral Flow Assay
Technical Report,01 Jan 2019,31 May 2020
CCDC Army Research Laboratory Adelphi United States
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The lateral flow immunoassay LFA is a robust, rapid point-of-care test used to detect specific molecules in biological samples. They hold significant advantages for use in the US Army, including monitoring of warfighter health and performance as well as providing the ability to test for hazardous foreign substances in the field. The traditional LFA uses antibodies as biorecognition elements, which can limit the shelf-life and operational functionality of the assay due to the poor thermostability exhibited by antibodies. Additionally, significant time is required to manufacture antibodies. As an alternative for antibodies, peptides are capable of comparable performance but with added stability and ease of production. In this report we investigate some of the considerations required to facilitate incorporation of peptides into the LFA using streptavidin-binding-peptide as the model reagent.
- Medicine and Medical Research