The Urinary Fungal Mycobiome and Host Responses in Patients with Interstitial Cystitis
Technical Report,15 Jul 2017,14 Jan 2020
Cedars-Sinai Medical Center Los Angeles United States
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The overall hypothesis of the original project was that uncultivated commensal microbial communities and their interactions with the host are associated with the development of ICBPS and that the resultant inflammatory host responses, assessed in the urine and blood, create a diagnostic signature specific to ICBPS. We used two complementary approaches to test this hypothesis 1 state-of-the-art next-generation sequencing NGS resources in microbial community profiling to define fungal and bacterial microbiota of ICBPS patients in comparison to normal subjects, and 2 proteomic analysis of urine and blood from ICBPS subjects to identify a specific signature correlating with disease phenotype and severity.We have made significant progress in each of our aims, identifying clinically relevant microbial biomarkers that 1 correlate with ICBPS, 2 distinguish bladder pain from alternate patterns of pelvic pain refractory to interstitial cystitis treatments and 3 associate with specific inflammatory profiles. We have identified symptom-specific urinary microbial profiles, urotypes, associated with bladder and pelvic pain that are useful as diagnostic biomarkers. In addition, proteomic profiling of ICBPS subgroups revealed distinct inflammatory associations. These results indicate promising pathways for further research into the mechanisms behind ICBPS and potential treatment modalities.
- Medicine and Medical Research