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A 3D Bioprinted Model for the Study of Premalignant Disease

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Technical Report,01 May 2016,30 Apr 2019

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University of Pittsburgh Pittsburgh United States

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This proposal involved a multidisciplinary team including a surgical oncologist, a mammary gland biologist, a biomedical engineer, and a cancer biologist. We hypothesized that a novel in vitro 3D bioprinted model of premalignant breast cells growing within a breast ductal system will represent a faithful representation of premalignant progression in vitro and will be an outstanding model for identify markers of low-risk premalignant disease which doesnt require treatment. To build the model, we first quantified murine mammary gland development, finding strain dependent differences highlighting a genetic component. We developed a proof-ofprinciple3D printing technology to print a mammary gland, and comprehensively developed a 3D printed ductal system with perfusion to study breast cells in their 3D environment. We have iteratively improved the quality and fidelity of the 3D printed ductal system composed of collagen and other ECM proteins and examined growth and behavior of breast cells in this 3D mammary duct in vitro. Further development of this model may help in understanding premalignant forms of breast cancer.

Subject Categories:

  • Medicine and Medical Research
  • Anatomy and Physiology

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