Instrumentation for Single Molecule Fluorescence Lifetime Imaging in Living Systems
Technical Report,01 Jul 2018,30 Jun 2019
WASHINGTON STATE UNIVERSITY PULLMAN United States
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Developed was a confocal attachment, that incorporated a fast piezo scanner, a high NA microscope objective, and a picosecond super continuum laser that was interfaced with an existing Guide Star microscope and TTTR-TCSPC instrumentation to create a fluorescence lifetime imaging FLIM capability with single molecule sensitivity along with its current state-of-the-art super-resolution capability. This instrument was calibrated to account for the absolute number of photons emitted from an individual probe molecule giving rise to the real-time quantum calibration of probes in situ.