Accession Number:

AD1103122

Title:

Instrumentation for Single Molecule Fluorescence Lifetime Imaging in Living Systems

Descriptive Note:

Technical Report,01 Jul 2018,30 Jun 2019

Corporate Author:

WASHINGTON STATE UNIVERSITY PULLMAN United States

Personal Author(s):

Report Date:

2019-09-12

Pagination or Media Count:

9.0

Abstract:

Developed was a confocal attachment, that incorporated a fast piezo scanner, a high NA microscope objective, and a picosecond super continuum laser that was interfaced with an existing Guide Star microscope and TTTR-TCSPC instrumentation to create a fluorescence lifetime imaging FLIM capability with single molecule sensitivity along with its current state-of-the-art super-resolution capability. This instrument was calibrated to account for the absolute number of photons emitted from an individual probe molecule giving rise to the real-time quantum calibration of probes in situ.

Subject Categories:

  • Optics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE