Persufflation of Composite Tissue Transplants
Technical Report,01 Sep 2017,31 Dec 2019
Trustees of Boston University Boston United States
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The objective of this project was to test whether persufflation--perfusion with gaseous oxygen--can extend the time that a composite tissue transplant can be preserved in the cold. This work used the rat groin flap microsurgical model, in which the femoral vessels serve as the vascular pedicle. Our results showed that static cold storage in University of Wisconsin organ preservation solution for twenty-four hours was insufficient to maintain the viability of flaps seven days post-implant. Persufflation for twenty-four hours neither improved nor worsened flap viability. Shortening the storage time to fifteen hours also did not alter the effect of persufflation on flap viability. No clear differences in tissue histology were observed between explants of cold-stored versus persufflated flaps.
- Medicine and Medical Research