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Metabolic Alterations in the Erythrocyte During Blood-Stage Development of the Malaria Parasite

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Journal Article - Open Access

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The Henry M. Jackson Foundattion for the Advancement of Military Medicine, Inc. Bethesda United States

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Human blood cells erythrocytes serve as hosts for the malaria parasite Plasmodium falciparum during its 48-h intraerythrocytic developmental cycle IDC. Established in vitro protocols allow for the study of hostparasite interactions during this phase and, in particular, high-resolution metabolomics can provide a window into hostparasite interactions that support parasite development. Uninfected and parasite-infected erythrocyte cultures were maintained at 2 percent haematocrit for the duration of the IDC, while parasitaemia was maintained at 7 percent in the infected cultures. The parasite-infected cultures were synchronized to obtain stage-dependent information of parasite development during the IDC. Samples were collected in quadruplicate at six time points from the uninfected and parasite-infected cultures and global metabolomics was used to analyze cell fractions of these cultures.

Subject Categories:

  • Medicine and Medical Research
  • Pharmacology

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