Metabolic Alterations in the Erythrocyte During Blood-Stage Development of the Malaria Parasite
Journal Article - Open Access
The Henry M. Jackson Foundattion for the Advancement of Military Medicine, Inc. Bethesda United States
Pagination or Media Count:
Human blood cells erythrocytes serve as hosts for the malaria parasite Plasmodium falciparum during its 48-h intraerythrocytic developmental cycle IDC. Established in vitro protocols allow for the study of hostparasite interactions during this phase and, in particular, high-resolution metabolomics can provide a window into hostparasite interactions that support parasite development. Uninfected and parasite-infected erythrocyte cultures were maintained at 2 percent haematocrit for the duration of the IDC, while parasitaemia was maintained at 7 percent in the infected cultures. The parasite-infected cultures were synchronized to obtain stage-dependent information of parasite development during the IDC. Samples were collected in quadruplicate at six time points from the uninfected and parasite-infected cultures and global metabolomics was used to analyze cell fractions of these cultures.
- Medicine and Medical Research