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Dissecting neuronal participation to focal epileptic events in vivo

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Technical Report,30 Sep 2018,29 Sep 2019

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Boston VA Research Institute, Inc. WEST ROXBURY United States

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The high prevalence of epilepsy among veteran populations with traumatic brain injury TBI makes epilepsy one of the congressionally directed topicareas. In previous studies, the electroencephalography EEG recording at the cortical surface during TBI induced epileptic seizures revealed hypersynchronousepileptic bursts, whereas single cell recordings found heterogeneous neuronal spikes during the hyper synchronous EEG burstsTruccolo et al., 2011. To define the correlation between the EEG and single neuron activity and to determine how different cell types participate in seizureevents, we monitor the individual activity of a large number of neurons in vivo using 2 photon microscopy. As a model of the long term effects of TBI, weinject tetanus toxin TeT into the visual cortex of mice to induce seizures. The activity dependent calcium indicator GCamp6, which in our caseis expressed in selective neurons by gene modification, or in all neurons by virus infection, reports the activity of individual neurons. Severaltypes of neurons from multiple layers of the visual cortex are recorded at several time points. The experimental timeline is shown in Figure 1.

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  • Anatomy and Physiology

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