Programmable Synthesis of DNA Nanostructures for Spatial and Temporal Control
Technical Report,01 Jun 2016,30 Nov 2019
Harvard University Cambridge United States
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Structural and dynamic DNA nanotechnology works have demonstrated the potential of harnessing the predictable and programmable base pairing of DNA to create self-assembled 3D nanostructures, design molecular walkers, and perform logic computation. However, scaling these capabilities has continuously required the development of novel and generalizable molecular mechanisms. We developed the Primer Exchange Reaction PER method and related technologies for nanostructure design and assembly that together address these scalability challenges, as well as open up the potential application space for recording and controlling spatio-temporal interactions using DNA circuitry. We showed how PER cascades, which autonomously synthesize single-stranded DNA sequences, can be used to perform computation, record the order of molecular events, synthesize a DNAzyme only in the presence of an input RNA. be used to create strands for a DNA origami nanostructure, and utilized to amplify fluorescence signal for imaging fixed DNA, RNA, and protein targets in situ. These demonstrations show just a few of the many applications of the PER technology for enhancing our ability to manipulate and record biological substrates.
- Optical Detection and Detectors
- Genetic Engineering and Molecular Biology