Accession Number:

AD1099404

Title:

Ultra High-Throughput Drug Screen for Lipid-Regulated Ion Channels

Descriptive Note:

Technical Report,30 Sep 2018,29 Sep 2019

Corporate Author:

Scripps Research Institute Jupiter United States

Personal Author(s):

• Hansen,Scott B.

Report Date:

2019-10-01

Pagination or Media Count:

9.0

Abstract:

A dearth of truly high throughput mechanisms to screen ion channels has hampered the development of pharmacological tools and therapeutics for effectively treating diseases involving ion channels channelopathies. Ion channels are drug targets for pain, cardiac function, and neurological disorders. We have developed a soluble assay for ion channels using a phosphatidylinositol 4,5-bisphosphate fluorescentprobe FL-PIP2 and detergent purified two pore domain potassium K2P and inward rectifying potassium Kir channels fused with a nano luciferece luminescent protein n-Luc. The assay works by bioluminescence energy transfer BRET.

Descriptors:

• drugs
• assays
• potassium
• proteins
• energy transfer

Subject Categories:

• Medicine and Medical Research
• Biochemistry
• Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE