Accession Number:

AD1096320

Title:

Cell Response Determinants in Laser-Induced Thermal Impacts

Descriptive Note:

Technical Report,16 Sep 2016,15 Dec 2018

Corporate Author:

MEDIZINISCHES LASERZENTRUM LUEBECK Luebck Germany

Personal Author(s):

• Miura,Yoko
• Brinkmann,Ralf

Report Date:

2019-05-06

Pagination or Media Count:

33.0

Abstract:

In contrast to exogenous determinant of cell death temperature x time following thermal stimulation, endogenous determinants turned out to be complexed and highly-regulated involving different proteins and molecules. Hsp70, which had been assumed to be one of the dominant determinants, also seems to be regulated by other different molecules such as VEGF and Thioredoxin, and calcium signaling appeared to be one of the key factors. We have established a real-time temperature measurement method for in-vitro cultures using optoacoustic technique. Using this method, mechanisms of temperature-resolved cell responses and determinants of subsequent cell functions could be investigated more in detail.

Descriptors:

• cultured cells
• growth factors
• ophthalmology
• laser beams
• proteins
• culture techniques
• epithelial cells
• cell physiological processes
• laser applications
• accuracy
• measurement

Subject Categories:

• Lasers and Masers
• Plasma Physics and Magnetohydrodynamics
• Fusion Devices (Thermonuclear)
• Lasers and Masers
• Plasma Physics and Magnetohydrodynamics
• Fusion Devices (Thermonuclear)

Distribution Statement:

APPROVED FOR PUBLIC RELEASE