Accession Number:

AD1095909

Title:

Strategies to Improve Chronic Nerve Regeneration and Target Reinnervation

Descriptive Note:

Technical Report,30 Sep 2017,29 Sep 2018

Corporate Author:

Johns Hopkins University Baltimore United States

Personal Author(s):

Report Date:

2018-10-01

Pagination or Media Count:

23.0

Abstract:

This study was designed to examine the effects of delivery of GMF-beta to denervated nerve to preserve the reactive state of Schwann cells and delivery of IGF-1 to denervated muscle to preserve muscle bulk and reinnervation capacity using a model of chronic nerve injury. We were able to complete the aim focusing on IGF-1 and demonstrated that IGF-1 encapsulated into slow release nanoparticles is able to preserve muscle bulk during chronic denervation and keeps it ready for reinnervation. We did have some technical issues with the GMF-beta delivery as the commercial sources for GMF-beta were unreliable. Recently we were able to make our own GMF-beta and we are currently evaluating the effects on chronically denervated Schwann cells. As a backup we also developed a non-viral gene delivery approach. We plan to combine these two approaches in the second half of Year 2 to examine the effects on functional recovery after chronic nerve injury.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE