Strategies to Improve Chronic Nerve Regeneration and Target Reinnervation
Technical Report,30 Sep 2017,29 Sep 2018
Johns Hopkins University Baltimore United States
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This study was designed to examine the effects of delivery of GMF-beta to denervated nerve to preserve the reactive state of Schwann cells and delivery of IGF-1 to denervated muscle to preserve muscle bulk and reinnervation capacity using a model of chronic nerve injury. We were able to complete the aim focusing on IGF-1 and demonstrated that IGF-1 encapsulated into slow release nanoparticles is able to preserve muscle bulk during chronic denervation and keeps it ready for reinnervation. We did have some technical issues with the GMF-beta delivery as the commercial sources for GMF-beta were unreliable. Recently we were able to make our own GMF-beta and we are currently evaluating the effects on chronically denervated Schwann cells. As a backup we also developed a non-viral gene delivery approach. We plan to combine these two approaches in the second half of Year 2 to examine the effects on functional recovery after chronic nerve injury.
- Medicine and Medical Research