Large Oncosomes: A Novel Liquid Biopsy for Genetic Profiling in Patients with Castration-Resistant Prostate Cancer
Technical Report,01 Sep 2017,31 Aug 2018
Cedars-Sinai Medical Center Los Angeles United States
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The overarching goal of my laboratory is to investigate whether circulating LO, a novel class of atypically large 1-10m diameter, bioactive extracellular vesicles EVs, which are released by highly invasive and metastatic amoeboid tumor cells in the plasma, and contain abundant RNA, miRNA, DNA, and protein cargo, report clinically relevant information and tumor-specific genomic alterations, thus representing a valuable alternative andor complement to other technologies proposed as a means of liquid biopsy. Collectively our findings indicate that DNA analyses of LO in blood plasma may provide a faithful representation of the genome of the tumor cells of origin. Because we have performed also comparative analysis with other EVs and shown that LO are a source of high quality and abundant DNA and they contain the whole genome of donor tumor cells, our overall objective is to test whether enumeration and genomic profiling of LO circulating in patient blood allow early detection of metastatic PC and identification of clinically significant PC-specific genomic aberrations, thereby overcoming the current limitations of the liquid biopsy.
- Medicine and Medical Research