Engineering a Minimal Competency Machinery for Mitochondria
Technical Report,01 Jun 2017,30 Nov 2018
University of Texas Dallas United States
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In our study, we will first engineer nucleic acid uptake for the organelle by introducing bacterial competency components targeted to the appropriate sub compartment of the mitochondrion. Using evolutionarily-related Gram-negative bacterial species as model systems, we will identify the minimal competency unit for the mitochondrion. We will then perform mitochondrial genome editing by introducing small guide RNAs and exogenous DNA sequences to the organelle for use by the CRISPRCas9 system. Together, we expect to develop a technique allowing for mitochondrial genome editing, which will lay the foundation for the future development of animals harboring human pathogenic mitochondrial DNA mutations. Having relevant and faithful disease models will provide new insights into the pathophysiology of disease, as well as the development of therapeutics.