Polycaprolactone-Collagen Composite Biomaterials for Mandible Regeneration
Technical Report,30 Sep 2017,29 Sep 2018
UNIVERSITY OF ILLINOIS URBANA United States
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Our primary objective is to demonstrate approaches to create mechanically robust, patient-customized biomaterials for large, load-bearing maxillofacial bone defects. To do this, we are developing approaches to integrate a biomolecule decorated collagen scaffold with micro-scale porosity into a mechanically-robust polymeric frame generated via 3D-printing with macro-porosity. Through the second year of this project we have completed the design, fabrication, as well as mechanical and in vitro osteogenesis testing of multiple elements of the final composite biomaterial design. We identified a PLA-collagen composite that could support robust mesenchymal stem cell MSC viability, osteogenic differentiation, and new mineral synthesis while rendering it shape-fitting to improve conformal contact with the wound margin. We have advanced multiple strategies to incorporate biomolecular signals into the collagen scaffold via transient sequestration, covalent attachment, and through included zinc ions that enhance osteogenic activity. Ongoing efforts are completing biomolecule incorporation and release experiments to facilitate long-term greater than 7 day biomolecule bioavailability via mineral based factor sequestration chemistries. Our efforts are essential for our goal to identify a shelf-stable, patient customizable biomaterial that can be seeded with autologous MSCs to regenerate large CMF bone defects.
- Medicine and Medical Research