Accession Number:

AD1095565

Title:

Autophagosomal Sequestration of Mitochondria as an Indicator of Antiandrogen TherapyResistance of Prostate Cancer (PCa)

Descriptive Note:

Technical Report,15 Sep 2017,14 Sep 2018

Corporate Author:

University of Texas MD Anderson Cancer Houston United States

Personal Author(s):

Report Date:

2018-10-01

Pagination or Media Count:

13.0

Abstract:

Purpose We are investigating if sequestration of metabolically dysfunctional mitochondria by the autophagosomes mitophagy imparts anti-androgen resistance and if this phenomenon can be applied in circulating tumor cells in patient blood samples as a biomarker for development of drug resistance. Method Effects of the anti-androgen enzalutamide on the autophagy and mitophagy of androgen-dependent LNCaP and independent C4-2 and CWR22v1 cells are studied first. Autophagy is monitored by fluorescence of cells with anti-LC3B antibody. Cellular fluorescence due to Mitosox dye oxidation is used to identify mitochondria producing high superoxide O2-.

Subject Categories:

  • Medicine and Medical Research
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE