Targeted Sequencing Toward Precision Wound Care
Technical Report,30 Sep 2018,29 Sep 2019
Lawrence Livermore National Laboratory Livermore United States
Pagination or Media Count:
Novel methods are urgently needed to support precision wound infection care. A lack of diagnostic information limits the ability to act and intervene in the context of wound infection. The effort described in this report develops and tests a targeted sequencing platform for measuring microbial genomic determinants critical to wound healing. We applied a custom bioinformatics approach to design DNA probes for capture of sample sequence that indicates the species present for a select set of wound-associated pathogens, in addition to genomic determinants for virulence and antimicrobial resistance. The panel was tested against control reference microbial genomes in samples within simulated human background. Sensitive, specific detection via the targeted sequencing panel was demonstrated in these validation samples for microbial species identity and virulence gene profile. The potential benefits of this developed panel to wound infection care in the Military Health System are numerous and wide ranging, including more timely evaluation of infection properties.
- Genetic Engineering and Molecular Biology