Auditory Cortical Network Changes in Tinnitus
Technical Report,01 Sep 2016,28 Feb 2018
University of Maryland, College Park College Park United States
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We proposed to study the impact of noise trauma on neuronal circuits in the auditory cortex of mice. During the award period, we have demonstrated progress in the following areas as related to the Statement of Work. First, we have used 2-photon laser scanning microscopy 2PLSM techniques to measure population activity of cortical Layer 23 neurons in control animals. We found that neuronal populations in L23 display a sparse representation of sound frequency in which neurons are selective, sparse, and perhaps carry increasingly higher amounts of stimulus information as activity moves through cortical layers. Second, we have enhanced our capabilities for behavioral testing by building hardware and software for two additional testing stations. Third, using 2PLSM techniques we have measured neuronal activity in specific subpopulations of inhibitory neurons in L23 i.e., those expressing parvalbumin, PV, somatostatin, SOM, vasoactive intestinal peptide, VIP. We have characterized gap detection behavior of multiple cohorts of mice and obtained stable baseline data in all of them. Our noise overexposure protocol has not produced animals that display behavioral evidence of chronic tinnitus. Future work will aim at using operant conditioning paradigms to test for behavioral evidence of chronic tinnitus.
- Anatomy and Physiology
- Medicine and Medical Research