Accession Number:

AD1095222

Title:

Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines

Descriptive Note:

Technical Report,01 Sep 2018,31 Aug 2019

Corporate Author:

University of Rhode Island Kingston United States

Personal Author(s):

Report Date:

2019-09-01

Pagination or Media Count:

24.0

Abstract:

The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive T and B cell responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the third project year, we performed genomic characterization of study subjects using molecular HLA typing, RNA-seq of unfractionated PBMC, and single-cell RNA-seq of PBMC. Genomic data were analyzed for associations with vaccine regimen and measures of vaccine immunogenicity. Preliminary findings from these analyses are being explored further.

Subject Categories:

  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE