Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines
Technical Report,01 Sep 2018,31 Aug 2019
University of Rhode Island Kingston United States
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The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive T and B cell responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the third project year, we performed genomic characterization of study subjects using molecular HLA typing, RNA-seq of unfractionated PBMC, and single-cell RNA-seq of PBMC. Genomic data were analyzed for associations with vaccine regimen and measures of vaccine immunogenicity. Preliminary findings from these analyses are being explored further.
- Medicine and Medical Research