Stroma-Based Prognosticators Incorporating Differences between African and European Americans
Technical Report,30 Sep 2015,29 Sep 2019
University of California Irvine United States
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Our objective was to use standard Formalin-fixed Paraffin-embedded FFPE tissue to identify RNAs in prostate tumor-adjacent stroma that distinguish between cancers in European Americans EA and African Americans AA, and to determine which fraction of these differences were associated with DNA methylation andor protein changes. Gleason Score, chemical relapse status, and approximate age, were used to match AA with EA patients. We found striking differences in interferon stimulated genes between AA and EA tumor-adjacent stroma, of potential value as prognostic and therapeutic targets. To establish an in vitro test-bed for these observations, it became necessary to developed a valuable resource of new normal and cancer-associated fibroblast cell cultures. The advances made in this project are the basis of a funded five year follow-up grant.
- Medicine and Medical Research