A Multidisciplinary Approach to Study the Role of the Gut Microbiome in Relapsing and Progressive MS
Technical Report,30 Sep 2015,29 Sep 2019
University of California San Diego San Diego United States
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Multiple sclerosis MS is a chronic, inflammatory disease of the central nervous system CNS and a common cause of progressive neurological disability in young adults. The typical disease course 85 of patients is relapsing or bout-onset MS RMS but 15 of patients do not have relapses and experience progression of disability from disease onset or primary progressive form of MS PPMS. We hypothesize that the gut microbiota in RMS and PPMS patients is different, thus potentially influencing disease course. In this project we recruited 381 subjects 149 RMS patients, 84 PPMS and 148 healthy and performed fecal bacterial DNA by 16S ribosomal RNA gene sequencing, and high-resolution HLA typing. In addition, we transferred microbiota from RMS n2 and PPMS n2 patients into germfree mice before inducing experimental allergic encephalomyelitis EAE. Despite the heterogeneous and modest sample size, we observed increased frequency of Enterobacteriaceae in RRMS patients and a reduction of Prevotella Copri in PPMS subjects. Transplantation of fecal microbiota from RMS patients into germ free mice resulted in milder neurological disability EAE compared to mice receiving microbiota from PPMS patients. While results need to be replicated, this suggests that gut microbiota from PPMS is less inflammatory than that of RMS subjects, thus shedding some light into disease pathogenesis.
- Medicine and Medical Research