Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1
Journal Article - Open Access
NATIONAL INST OF ALLERGY AND INFECTIOUS DISEASES HAMILTON MT HAMILTON
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A novel human coronavirus that is now named severe acute respiratory syndrome coronavirus 2 SARS-CoV-2 formerly called HCoV-19 emerged in Wuhan, China, in late 2019 and is now causing a pandemic.1 We analyzed the aerosol and surface stability of SARS-CoV-2 and compared it with SARS-CoV-1, the most closely related human coronavirus.2We evaluated the stability of SARS-CoV-2 and SARS-CoV-1 in aerosols and on various surfaces and estimated their decay rates using a Bayesian regression model see the Methods section in the Supplementary Appendix, available with the full text of this letter at NEJM.org. SARS-CoV-2nCoV-WA1-2020 MN985325.1 and SARS-CoV-1Tor2 AY274119.3 were the strains used. Aerosols5 micron containing SARS-CoV-2 105.25 50tissue-culture infectious dose TCID50 per milliliteror SARS-CoV-1 106.75-7.00 TCID50 per milliliterwere generated with the use of a three-jet Collison nebulizer and fed into a Goldberg drum to create an aerosolized environment. The inoculum resulted in cycle-threshold values between20 and 22, similar to those observed in samples obtained from the upper and lower respiratory tract in humans.
- Medicine and Medical Research