Rational Therapies for Diffuse-Type Gastric Cancer
Technical Report,15 Aug 2017,14 Aug 2018
The Regents of the University of California San Francisco United States
Pagination or Media Count:
We have collected a panel of 7 gastric cell line models MKN7, AGS, KATO-III, NCI-SNU-1, NCI-SNU-5, NCI-SNU16 and NCI-N87. Using data available through CCLE and Cosmic Databases Garnett, 2012 no. 76 Barretina, 2012 no. 1, we determined the copy number and mutational status of CDH1 and identified a single base pair insertion in AGS cells prematurely terminates CDH1 in exon 12. We have designed and constructed CRISPR reagents to correct this mutation and restore CDH1 function. This isogenic cell line pair will be used to investigate the signaling networks in CDH1-deficient cells compared to CDH1-proficient lines.
- Medicine and Medical Research