Nanoink Printed Amperometric Immunosensor for Rapid and Inexpensive Screening of Tuberculosis
Technical Report,01 Jun 2018,31 Oct 2018
University of Washington Seattle United States
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The project aims to develop a point-of-care diagnostic platform for tuberculosis diagnosis. The sensor detects Mycobacterium tuberculosisMTB H37Ra strain cells and MPT-64 antigen spiked in human sputum samples. Single walled carbon nanotubes are used as a sensing element for detection of target analytes. In the project period, various SWCNTs-based immunosensors were designed and fabricated. The fabrication and detection protocol was optimized. For specific detection, antibodies to MPT-64 were generated and characterized. Through affinity purification, the antibodies became more sensitive and specific to target analytes. Using the sensor, the detection limit was 106CFUmL for BCG and 100 ngmL for MPT-64. In the next project period, the detection protocol will be further optimized to achieve detection limit of 103 CFUmL or equivalent concentration to MPT-64.
- Medicine and Medical Research