Persufflation of composite tissue transplants
Technical Report,01 Sep 2017,31 Aug 2018
Trustees of Boston University Boston United States
Pagination or Media Count:
The objective of this project is to test whether persufflationperfusion with gaseous oxygencan extend the time that a composite tissue transplant can be preserved in the cold. This work uses the rat groin flap microsurgical model, in which the femoral vessels serve as the vascular pedicle. Experiments to date have shown that static cold storage in University of Wisconsin organ preservation solution for twenty-four hours is insufficient to maintain the viability of flaps seven days post-implant. Persufflation for twenty-four hours led to better integration of implants, but was not equivalent to unpreserved implants i.e., immediate replantation. Ongoing studies will optimize the persufflation procedure by varying the persufflation time, the perfusion direction, and the gas pressure.
- Medicine and Medical Research