Direct Imaging of Modulatory Neurotransmitters Using Synthetic Nanosensors to Understand and Treat Parkinsons Disease: Early Investigator Award
Technical Report,15 Jul 2018,14 Jul 2019
The Regents of the University of California BERKELEY United States
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Nanosensors for the optical detection of dopamine in brain slice has been validated in wildtype C57C6 mice as well as a Parkinsons model mouse. Stereotaxic LPS injections into the substantia nigra of mice selectively kills dopaminergic neurons and produces a Parkinsons phenotype. This is confirmed using immunohistochemistry and cell death stains in fixed slice. Using nanosensors, endogenous dopamine release in striatal brain slice is triggered by electrical stimulation and quantified using microscopy. We have established the workflow for this procedure and have prepared a population of Parkinsons and wildtype mice to image in the coming quarter.
- Medicine and Medical Research