Discovery and Characterization of PRCAT47: A Novel Prostate Lineage and Cancer-Specific Long Noncoding RNA
Technical Report,01 Jul 2016,30 Jun 2019
University of Michigan Ann Arbor United States
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The overall goal of this project is to characterize the functioning mechanism, utility and therapeutic potential of the biomarker PRCAT47 in prostate cancer. During this second period, we investigated the regulatory effect of PRCAT47 on androgen receptor ARtranscript stability. We also interrogated the role of PRCAT47 in regulating HuRTIAR-targeted mRNAs. Utilizing a novel RNA in situ hybridization technology optimized within the first period which allows us to assess PRCAT47 expression in prostate tissues, we have not evaluatedPRCAT47 expression in prostate cancer tissue microarrays by RNA-ISH and demonstrate an association with prostate cancer progression. Finally, we also assessed the therapeutic potential of PRCAT47 using cell line models and patient derived organoid cultures.
- Medicine and Medical Research