Evaluation of the Physiological Challenges in Extreme Environments: Implications for Enhanced Training, Operational Performance and Sex-Specific Responses
Technical Report,30 Sep 2017,29 Sep 2018
The University of Montana Missoula United States
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The specific aim of the second and third segments of this project series were to determine the impacts of environmental conditions on specific markers of exercise training response andor acclimation. In the second year FY17, we initiated the current training program in untrained males. This was continued for the first few months of FY18. The project was revised in late 2018 December to include females. Overall, untrained males n36 and females n24 served as study participants. The males were stratified across three training environments 1 control approximately 20 degrees C, 30 percent RH, 2 heat approximately 33 degrees C, 30 percent RH, or 3 cold approximately 7 degrees C, 30 percent RH environments n12 in each environment. The untrained females were stratified across the control and heated environments n12 in each group. Daily training was prescribed for a period of 14 days. Following a controlled diet and exercise plan the day before and after overnight fast participants reported to the laboratory in the early morning hours. A muscle biopsy was obtained from the vastus lateralis randomized, counter-balanced leg before starting exercise Pre days 1 and 15. Participants then exercised for 60 minutes at 60 percent of maximal aerobic capacity in one of the above-described environments. At the conclusion of the exercise trial, participants recovered seated rest, under normal laboratory conditions for four hours before a final muscle biopsy was taken days 1 and 15. The additional four hours was necessary for the genes of interest to peak following exercise as previously described. Data was analyzed using a mixed design ANOVA with repeated measures between the training groups, sex and across time time x trial x sex. A probability of type I error of less than 5 percent was considered significant p 0.05.
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