Acute Pancreatitis as a Model to Predict Transition of Systemic Inflammation to Organ Failure in Trauma and Critical Illness
Technical Report,22 Sep 2014,21 Sep 2018
UNIVERSITY OF PITTSBURGH PITTSBURGH United States
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Trauma, extensive burns, bacterial infections, and acute pancreatitis AP are common conditions of tissue injury and immune system activation that can result in the systemic inflammatory response syndrome SIRS. Surprisingly, about half of the patients with SIRS quickly recover, while the others develop a multiorgan dysfunction syndrome MODS. SIRS and MODS do not occur immediately SIRS evolves over a 4-12 hour period, while MODS evolves over12-24 hours. Vascular leak syndrome VLS is a critical component of the transition from SIRS to MODS. Understanding the mechanism by which SIRS triggers VLS and progresses to MODS is critical to correctly model disease course thereby aiding in treatment of patients. We tested the effect of severe acute pancreatitis patient serum on the viability of human vascular endothelial cells grown in a monolayer and evaluated potential cell death mechanisms. Serum from enrolled subjects was also tested for potential biomarkers in order to better understand course from SIRS to organ failure OF. Our data shows that main modes of cell death are necrosis and autophagy and that multiple mechanisms are involved in course to OF.
- Medicine and Medical Research