Discovery and Characterization of Functional Breast Cancer Microproteins
Technical Report,01 Aug 2018,31 Jul 2019
Salk Institute for Biological Studies La Jolla United States
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There were two significant findings during the research period. First, using antibodies we raised against the TINCR microprotein TINCR-MP, we validated the translation of TINCR-MP into a stable microprotein. Though we had detected a small open reading frame smORF on TINCR this was the first confirmation of the TINCR microprotein. We also used this antibody to show that changes in TINCR microprotein levels readily be detected, which is necessary for our biological studies looking at the consequences of TINCR-MP on breast cancer cells. Second, we detected thousands of smORFs in MCF-7 and MD-MB-231 breast cancer cell lines, with approximately one hundred smORFs showing a 3-fold or more difference between these cell lines. Identifying these smORFs will help determine whether any of these genes contribute to the cancer phenotypes of these cell lines. These findings further validate our methodology and we are in an ideal position to complete the project in the allotted time.
- Medicine and Medical Research