Mammary Tissue Host-Microbiome Interactions in Breast Cancer Development
Technical Report,30 Sep 2018,29 Sep 2021
Pepperdine University Malibu United States
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Through this project we will determine the role of the mammary tissue microbiome in breast cancer development using 16S ribosomal RNA sequencing and dual-transcriptomic sequencing. In the first year of this project we have selected and received 165 samples from the Susan G. Komen and Indiana University Simon Cancer Center Tissue Banks. Isolation of DNA for 16S rRNA sequencing is well underway and we anticipate submission of DNA for sequencing in December 2019 and results from this portion of this project in March 2020. We are currently analyzing pilot dual-RNA sequencing data, which will inform any procedural modifications we should make to our RNA isolation protocols. We will use the 16S data to inform the depth of coverage necessary to conduct this type of novel transcriptomic sequencing. As such, the RNA sequencing analysis will be delayed until late Springearly Summer 2020. Thus far, the project is well underway. Results from this work will be key in characterizing host-microbiome cross-talk in the pathogenesis of breast tumor development.
- Medicine and Medical Research