The Urinary Fungal Mycobiome and Host Responses in Patients with Interstitial Cystitis
Technical Report,15 Jul 2018,14 Jul 2019
Cedars-Sinai Medical Center Los Angeles United States
Pagination or Media Count:
We have completed recruitment of 200 subjects, have identified distinctive bacterial community types with a relationship to dysbiosis, and developed new techniques for identifying both bacterial and fungal species. We have successfully extracted both fungal and bacterial DNA from the catheterized urine samples and confirmed this through both quantitative polymerase chain reaction qPCR and gel electrophoresis. We have refined classification of bacterial communities found in both controls and individuals diagnosed with Interstitial Cystitis and confirmed the connection between specific community types and pelvic paindisease. We have developed specific probes that allow us to complete fungal analysis. We have developed a more sophisticated approach to biostatistical analysis and are currently completing, proteomics, analysis and Multiple Reaction Monitoring validation.