Defining Alpha and Beta-Cell Crosstalk for the Treatment and Prevention of Diabetes
Technical Report,15 Jun 2018,14 Jun 2019
CORNELL UNIV ITHACA NY ITHACA United States
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During this funding period we have bred up mice for study, have begun to enroll mice into study and generate samples for analysis in both aims 1 and 2. The primary finding to report at this time is our work in sham and VSG-operated MIPCre and MIPCre- mice that do not contain the floxed GLP-1R allele. These additional control groups demonstrate that the MIPCre allele does not alter our glucoregulatory findings after VSG in mice. In addition, as a complementary approach, we have developed a new single cell RNA-sequencing method for the study of islets. We have used this approach to study islets treated with and without a GLP-1 receptor agonist from healthy human donors. These data reveal that GLP-1 receptor signaling in human islets increases alpha cell expression of the prohormone convertase needed for GLP-1 production, demonstrating that our model of beta cell GLP-1R function has translational relevance in humans.
- Medicine and Medical Research