Characterization of Clustered CTCs to Eliminate Breast Cancer Metastasis
Technical Report,01 Jun 2018,31 May 2019
Case Western Reserve University Cleveland United States
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This program examines the role of platelets and IL11 in breast cancer tumor cell clustering and metastasis in the lung. In year 1, we determined that only 5 days of IL11 treatment to C57BL6mice resulted in a 2-3-fold increase in platelet counts. In year 2, we created a murine model for metastatic breast cancer in C57BL6 mice and established facilities for the studies after a collaborator left. In year 3 we characterized the labeled E0771.LMB cells for these investigations. By cell sorting, we have prepared a homogenous population of cells with a high degree of Td-tomato expression an internal marker of the luciferase reporter that correlates with the degree of bioluminescence measured. With these cells, we now show critical proof of concept studies that platelet number alone greatly influences the degree of breast cancer tumor cell metastasis. Mice, made thrombocytopenic with anti-CD41, have less breast cancer cell seeding, smaller lung metastasis and fewer metastatic foci than mice with a normal platelet count. These studies prove the importance of platelet number in breast cancer metastasis and indicate that number alone at time of seeding influence metastasis.
- Medicine and Medical Research