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In-Vitro Experiments of Highly Dynamic Loading of Brain Slices and Cell Cultures

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Conference Paper

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59 MDW San Antonio United States

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Blast-induced traumatic brain injury bTBI is a signature wound affecting servicemembers in modern conflicts. When a blast wave generated by an improvised explosive device IED explosion waves propagate through the human head, it is hypothesized to cause direct mechanical damage to the brain tissue, but this mechanism and pathophysiology still remain poorly understood. One of the reasons for a poor understanding the bTBI mechanisms is the difficulty in reproducing the controlled blast-like pressure profiles pressure profiles with a sub-millisecond onset of high pressures 200-300 kPa within animals and celltissue cultures. To do so, researchers have had to rely upon experimental techniques such as shocktubes, blast-tubes, as well as Split-Hopkinson Bars 1-3. These methods are very expensive, require significant technical expertise, large laboratory spaces, and usually have limited control on the pressure profiles they can generate. The pressure profile generated by these methods is typically a single overpressure pulse which doesnt replicate the dynamics of the intracranial pressure ICP observed during a blast exposure 4. An ideal solution to this challenge is to create a completely controllable, portable, reproducible, and inexpensive method to generate blast-like pressure profiles upon living tissues and animal models and would be easily shared between laboratories.

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  • Medicine and Medical Research

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