Mechanical regulation of integrin conformation
Georgia Tech Research Corporation Atlanta United States
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The most important results of our studies have been published in two technical papers 1, 2 and a review paper 3, acknowledged the support of this grant. The first paper used a biomembrane force probe to characterize the bending and unbending conformational changes of single V3 integrins on living cell surfaces in real-time. We measured the probabilities of conformational changes, rates and speeds of conformational transitions, and the dynamic equilibrium between the two conformations, which were regulated by tensile force, dependent on the ligand, and altered by point mutations. These findings provide insights into how alpha-beta 3 acts as a molecular machine and how its physiological function and molecular structure are coupled at the single-molecule level.