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DURIP: Fluorescence Correlation Microscopy for Characterizing the Structural Dynamics of Biochemical Nanosystems

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Technical Report,12 Apr 2016,31 Jul 2016

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Rutgers, The State University of New Jersey Camden United States

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We propose to develop a confocal fluorescence correlation spectroscopy FCS to support research programs in the Department of Chemistry and Center for Computational and Integrative Biology at Rutgers University-Camden. The proposed FCS will be an upgrade of an existing confocal laser scanning microscope. FCS is a single-molecule detection method that measures fluctuations of fluorescence intensity in a sub-femtoliter volume, which detects kinetic and structural parameters, such as the diffusion time, number of molecules and dark states of fluorescently labeled molecules. Several research projects will be enhanced by the FCS that are either supported by Army or are relevant to DoD goals, including 1 Characterization of the assembly of switchable DNA nanostructures for developing regulatory biochemical reaction circuits 2 Develop an enzyme-powered nanomotor propelled by chemical fuels 3 Nanoparticle-mediated photoporation of cell membranes and the optical injection of exogenous matter 4 Study of protein and peptide aggregation 5 Structure and dynamics of protein unfolding in aqueous ionic liquid solutions. The proposed research projects with FCS will provide students opportunities to learn and practice the skills that are required in scientific research. This helps to maintain a sustainable future for STEM programs that develop students interests and skills for the future participation in scientific research. The program will also educate students about the Armys involvement of supporting fundamental science and engineering research as well as educational opportunities for students through the Army Educational Outreach Program AEOP and Department of Defense Graduate Fellowships. It will also provide research opportunities for under-representedlow-income high school students and undergraduates in the Camden area.

Subject Categories:

  • Biochemistry
  • Test Facilities, Equipment and Methods

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