Accession Number:

AD1081393

Title:

High Frequency Application of Nanosecond Pulsed Electric Fields Alters Cellular Membrane Disruption and Fluorescent Dye Uptake

Descriptive Note:

Technical Report,01 Jan 2013,31 Dec 2017

Corporate Author:

Human Performance Wing, Radio Frequency Bioeffects Branch JBSA Fort Sam Houston United States

Personal Author(s):

• Ibey,Bennett L.
• Steelman,Zachary A.
• Tolstykh,Gleb P.

Report Date:

2016-12-01

Pagination or Media Count:

8.0

Abstract:

Cells exposed to nanosecond-pulsed electric fields nsPEF exhibit a wide variety of nonspecific effects, including blebbing, swelling, intracellular calcium bursts, apoptotic and necrotic cell death, formation of nanopores, and depletion of phosphatidylinositol 4,5-biphosphate PIP2 to induce activation of the inositol trisphosphatediacylglycerol pathway.

Descriptors:

• electric fields
• microscopy
• cells(biology)
• membranes
• nanosecond time
• pulses

Subject Categories:

• Electricity and Magnetism
• Medicine and Medical Research
• Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE