Silver nanoparticle surface coatings effect on mitochondrial structure and function
Technical Report,01 Aug 2016,28 Feb 2019
Henry M. Jackson Foundation for the Advancement of Military Medicine Wright Patterson Air Force Base United States
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Silver nanoparticles AgNPs are widely used in numerous consumer products due to their antimicrobial properties. AgNPshave been shown to be toxic to human cells although this toxicity can be somewhat mitigated by coating the nanoparticlesurface to prevent Ag ion leakage. It has been suggested that Ag acts on mitochondria to induce the toxic response, however, acomprehensive examination of mitochondria has yet to be carried out. Mitochondria play many important roles in cells including energy production, regulation of reactive oxygen species, and apoptotic signaling. Mitochondria function is intimately tied to maintenance of mitochondrial structure which includes mitochondrial dynamics and ultrastructure. This study aims to examine mitochondria function and structure after the treatment of A549 cells with Ag coated with citrate, PVP, and BPEI to determine if the different surface coatings elicit different toxicological responses. Ag BPEI was the most toxic of the three coatings as it increased cell death, decreased mitochondrial membrane potential, and impacted mitochondrial ultrastructure. Ag citrate and Ag PVP had similar toxic responses to mitochondrial ultrastructure with Ag citrate being slightly more toxic as it entered cells sooner and caused an increase to mitochondrial area.
- Coatings, Colorants and Finishes