Virus adsorption to colloids in water: Interactions between bacteriophage MS2, kaolinite, and fiberglass
Technical Report,01 Oct 2017,21 Mar 2019
AIR FORCE INSTITUTE OF TECHNOLOGY WRIGHT-PATTERSON AFB OH WRIGHT-PATTERSON AFB United States
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Virus adsorption to colloidal particles is an important issue for wastewater treatment plants accepting biohazardous waste. Colloids impact the transport of viruses, and provide protection against oxidants and other destructive mechanisms. This study investigated adsorption of bacteriophage MS2 to colloidal suspensions of kaolinite KAO and fiber glass FG. Laboratory batch tests performed over a range of experimental conditions determined kinetic rate constants and characterized bond strength. Computational experiments assessed adsorption and aggregation of MS2. First order removal rate constants were faster than previously reported between 2.5 2.8 min-1 and 0.4 2.8 min -1 for KAO and FG respectively. Evidence of MS2 adsorption was collected with fluorescent and bright field microscopic images, showing MS2 clusters associated with colloidal particles. After two-hours the 55.2 - 80.8 of the adsorbed MS2 was tightly bound in the FG experiment and 54.8 - 87.9 in the KAO experiment. This implies MS2 has a stronger affinity for KAO than FG. MS2 aggregation was also observed experimentally and predicted with XDLVO models. These results show colloids can quickly and strongly attach to clusters of viruses, leading to significant implications for management of biohazardous wastes at water recovery facilities.
- Water Pollution and Control
- Physical Chemistry